Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry.
Identifieur interne : 001181 ( Main/Exploration ); précédent : 001180; suivant : 001182Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry.
Auteurs : Jun Chen [États-Unis] ; Susan M. Young ; Chris Allen ; Andrew Seeber ; Marie-Pierre Péli-Gulli ; Nicolas Panchaud ; Anna Waller ; Oleg Ursu ; Tuanli Yao ; Jennifer E. Golden ; J Jacob Strouse ; Mark B. Carter ; Huining Kang ; Cristian G. Bologa ; Terry D. Foutz ; Bruce S. Edwards ; Blake R. Peterson ; Jeffrey Aubé ; Margaret Werner-Washburne ; Robbie J. Loewith ; Claudio De Virgilio ; Larry A. SklarSource :
- ACS chemical biology [ 1554-8937 ] ; 2012.
Descripteurs français
- KwdFr :
- Cytométrie en flux (MeSH), Facteurs de transcription (antagonistes et inhibiteurs), Humains (MeSH), Inhibiteurs de protéines kinases (analyse), Protéines de Saccharomyces cerevisiae (antagonistes et inhibiteurs), Protéines à fluorescence verte (MeSH), Saccharomyces cerevisiae (effets des médicaments et des substances chimiques), Transduction du signal (effets des médicaments et des substances chimiques).
- MESH :
- analyse : Inhibiteurs de protéines kinases.
- antagonistes et inhibiteurs : Facteurs de transcription, Protéines de Saccharomyces cerevisiae.
- effets des médicaments et des substances chimiques : Saccharomyces cerevisiae, Transduction du signal.
- Cytométrie en flux, Humains, Protéines à fluorescence verte.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Protein Kinase Inhibitors.
- chemical , antagonists & inhibitors : Saccharomyces cerevisiae Proteins, Transcription Factors.
- chemical : Green Fluorescent Proteins.
- drug effects : Saccharomyces cerevisiae, Signal Transduction.
- Flow Cytometry, Humans.
Abstract
TOR (target of rapamycin) is a serine/threonine kinase, evolutionarily conserved from yeast to human, which functions as a fundamental controller of cell growth. The moderate clinical benefit of rapamycin in mTOR-based therapy of many cancers favors the development of new TOR inhibitors. Here we report a high-throughput flow cytometry multiplexed screen using five GFP-tagged yeast clones that represent the readouts of four branches of the TORC1 signaling pathway in budding yeast. Each GFP-tagged clone was differentially color-coded, and the GFP signal of each clone was measured simultaneously by flow cytometry, which allows rapid prioritization of compounds that likely act through direct modulation of TORC1 or proximal signaling components. A total of 255 compounds were confirmed in dose-response analysis to alter GFP expression in one or more clones. To validate the concept of the high-throughput screen, we have characterized CID 3528206, a small molecule most likely to act on TORC1 as it alters GFP expression in all five GFP clones in a manner analogous to that of rapamycin. We have shown that CID 3528206 inhibited yeast cell growth and that CID 3528206 inhibited TORC1 activity both in vitro and in vivo with EC(50)'s of 150 nM and 3.9 μM, respectively. The results of microarray analysis and yeast GFP collection screen further support the notion that CID 3528206 and rapamycin modulate similar cellular pathways. Together, these results indicate that the HTS has identified a potentially useful small molecule for further development of TOR inhibitors.
DOI: 10.1021/cb200452r
PubMed: 22260433
PubMed Central: PMC3331904
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry.</title><author><name sortKey="Chen, Jun" sort="Chen, Jun" uniqKey="Chen J" first="Jun" last="Chen">Jun Chen</name><affiliation wicri:level="1"><nlm:affiliation>Center for Molecular Discovery, University of New Mexico, Albuquerque, 87131, United States. juchen@salud.unm.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Molecular Discovery, University of New Mexico, Albuquerque, 87131</wicri:regionArea><wicri:noRegion>87131</wicri:noRegion></affiliation></author><author><name sortKey="Young, Susan M" sort="Young, Susan M" uniqKey="Young S" first="Susan M" last="Young">Susan M. Young</name></author><author><name sortKey="Allen, Chris" sort="Allen, Chris" uniqKey="Allen C" first="Chris" last="Allen">Chris Allen</name></author><author><name sortKey="Seeber, Andrew" sort="Seeber, Andrew" uniqKey="Seeber A" first="Andrew" last="Seeber">Andrew Seeber</name></author><author><name sortKey="Peli Gulli, Marie Pierre" sort="Peli Gulli, Marie Pierre" uniqKey="Peli Gulli M" first="Marie-Pierre" last="Péli-Gulli">Marie-Pierre Péli-Gulli</name></author><author><name sortKey="Panchaud, Nicolas" sort="Panchaud, Nicolas" uniqKey="Panchaud N" first="Nicolas" last="Panchaud">Nicolas Panchaud</name></author><author><name sortKey="Waller, Anna" sort="Waller, Anna" uniqKey="Waller A" first="Anna" last="Waller">Anna Waller</name></author><author><name sortKey="Ursu, Oleg" sort="Ursu, Oleg" uniqKey="Ursu O" first="Oleg" last="Ursu">Oleg Ursu</name></author><author><name sortKey="Yao, Tuanli" sort="Yao, Tuanli" uniqKey="Yao T" first="Tuanli" last="Yao">Tuanli Yao</name></author><author><name sortKey="Golden, Jennifer E" sort="Golden, Jennifer E" uniqKey="Golden J" first="Jennifer E" last="Golden">Jennifer E. Golden</name></author><author><name sortKey="Strouse, J Jacob" sort="Strouse, J Jacob" uniqKey="Strouse J" first="J Jacob" last="Strouse">J Jacob Strouse</name></author><author><name sortKey="Carter, Mark B" sort="Carter, Mark B" uniqKey="Carter M" first="Mark B" last="Carter">Mark B. Carter</name></author><author><name sortKey="Kang, Huining" sort="Kang, Huining" uniqKey="Kang H" first="Huining" last="Kang">Huining Kang</name></author><author><name sortKey="Bologa, Cristian G" sort="Bologa, Cristian G" uniqKey="Bologa C" first="Cristian G" last="Bologa">Cristian G. Bologa</name></author><author><name sortKey="Foutz, Terry D" sort="Foutz, Terry D" uniqKey="Foutz T" first="Terry D" last="Foutz">Terry D. Foutz</name></author><author><name sortKey="Edwards, Bruce S" sort="Edwards, Bruce S" uniqKey="Edwards B" first="Bruce S" last="Edwards">Bruce S. Edwards</name></author><author><name sortKey="Peterson, Blake R" sort="Peterson, Blake R" uniqKey="Peterson B" first="Blake R" last="Peterson">Blake R. Peterson</name></author><author><name sortKey="Aube, Jeffrey" sort="Aube, Jeffrey" uniqKey="Aube J" first="Jeffrey" last="Aubé">Jeffrey Aubé</name></author><author><name sortKey="Werner Washburne, Margaret" sort="Werner Washburne, Margaret" uniqKey="Werner Washburne M" first="Margaret" last="Werner-Washburne">Margaret Werner-Washburne</name></author><author><name sortKey="Loewith, Robbie J" sort="Loewith, Robbie J" uniqKey="Loewith R" first="Robbie J" last="Loewith">Robbie J. Loewith</name></author><author><name sortKey="De Virgilio, Claudio" sort="De Virgilio, Claudio" uniqKey="De Virgilio C" first="Claudio" last="De Virgilio">Claudio De Virgilio</name></author><author><name sortKey="Sklar, Larry A" sort="Sklar, Larry A" uniqKey="Sklar L" first="Larry A" last="Sklar">Larry A. Sklar</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22260433</idno><idno type="pmid">22260433</idno><idno type="doi">10.1021/cb200452r</idno><idno type="pmc">PMC3331904</idno><idno type="wicri:Area/Main/Corpus">001199</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001199</idno><idno type="wicri:Area/Main/Curation">001199</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001199</idno><idno type="wicri:Area/Main/Exploration">001199</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry.</title><author><name sortKey="Chen, Jun" sort="Chen, Jun" uniqKey="Chen J" first="Jun" last="Chen">Jun Chen</name><affiliation wicri:level="1"><nlm:affiliation>Center for Molecular Discovery, University of New Mexico, Albuquerque, 87131, United States. juchen@salud.unm.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Molecular Discovery, University of New Mexico, Albuquerque, 87131</wicri:regionArea><wicri:noRegion>87131</wicri:noRegion></affiliation></author><author><name sortKey="Young, Susan M" sort="Young, Susan M" uniqKey="Young S" first="Susan M" last="Young">Susan M. Young</name></author><author><name sortKey="Allen, Chris" sort="Allen, Chris" uniqKey="Allen C" first="Chris" last="Allen">Chris Allen</name></author><author><name sortKey="Seeber, Andrew" sort="Seeber, Andrew" uniqKey="Seeber A" first="Andrew" last="Seeber">Andrew Seeber</name></author><author><name sortKey="Peli Gulli, Marie Pierre" sort="Peli Gulli, Marie Pierre" uniqKey="Peli Gulli M" first="Marie-Pierre" last="Péli-Gulli">Marie-Pierre Péli-Gulli</name></author><author><name sortKey="Panchaud, Nicolas" sort="Panchaud, Nicolas" uniqKey="Panchaud N" first="Nicolas" last="Panchaud">Nicolas Panchaud</name></author><author><name sortKey="Waller, Anna" sort="Waller, Anna" uniqKey="Waller A" first="Anna" last="Waller">Anna Waller</name></author><author><name sortKey="Ursu, Oleg" sort="Ursu, Oleg" uniqKey="Ursu O" first="Oleg" last="Ursu">Oleg Ursu</name></author><author><name sortKey="Yao, Tuanli" sort="Yao, Tuanli" uniqKey="Yao T" first="Tuanli" last="Yao">Tuanli Yao</name></author><author><name sortKey="Golden, Jennifer E" sort="Golden, Jennifer E" uniqKey="Golden J" first="Jennifer E" last="Golden">Jennifer E. Golden</name></author><author><name sortKey="Strouse, J Jacob" sort="Strouse, J Jacob" uniqKey="Strouse J" first="J Jacob" last="Strouse">J Jacob Strouse</name></author><author><name sortKey="Carter, Mark B" sort="Carter, Mark B" uniqKey="Carter M" first="Mark B" last="Carter">Mark B. Carter</name></author><author><name sortKey="Kang, Huining" sort="Kang, Huining" uniqKey="Kang H" first="Huining" last="Kang">Huining Kang</name></author><author><name sortKey="Bologa, Cristian G" sort="Bologa, Cristian G" uniqKey="Bologa C" first="Cristian G" last="Bologa">Cristian G. Bologa</name></author><author><name sortKey="Foutz, Terry D" sort="Foutz, Terry D" uniqKey="Foutz T" first="Terry D" last="Foutz">Terry D. Foutz</name></author><author><name sortKey="Edwards, Bruce S" sort="Edwards, Bruce S" uniqKey="Edwards B" first="Bruce S" last="Edwards">Bruce S. Edwards</name></author><author><name sortKey="Peterson, Blake R" sort="Peterson, Blake R" uniqKey="Peterson B" first="Blake R" last="Peterson">Blake R. Peterson</name></author><author><name sortKey="Aube, Jeffrey" sort="Aube, Jeffrey" uniqKey="Aube J" first="Jeffrey" last="Aubé">Jeffrey Aubé</name></author><author><name sortKey="Werner Washburne, Margaret" sort="Werner Washburne, Margaret" uniqKey="Werner Washburne M" first="Margaret" last="Werner-Washburne">Margaret Werner-Washburne</name></author><author><name sortKey="Loewith, Robbie J" sort="Loewith, Robbie J" uniqKey="Loewith R" first="Robbie J" last="Loewith">Robbie J. Loewith</name></author><author><name sortKey="De Virgilio, Claudio" sort="De Virgilio, Claudio" uniqKey="De Virgilio C" first="Claudio" last="De Virgilio">Claudio De Virgilio</name></author><author><name sortKey="Sklar, Larry A" sort="Sklar, Larry A" uniqKey="Sklar L" first="Larry A" last="Sklar">Larry A. Sklar</name></author></analytic><series><title level="j">ACS chemical biology</title><idno type="eISSN">1554-8937</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Flow Cytometry (MeSH)</term><term>Green Fluorescent Proteins (MeSH)</term><term>Humans (MeSH)</term><term>Protein Kinase Inhibitors (analysis)</term><term>Saccharomyces cerevisiae (drug effects)</term><term>Saccharomyces cerevisiae Proteins (antagonists & inhibitors)</term><term>Signal Transduction (drug effects)</term><term>Transcription Factors (antagonists & inhibitors)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cytométrie en flux (MeSH)</term><term>Facteurs de transcription (antagonistes et inhibiteurs)</term><term>Humains (MeSH)</term><term>Inhibiteurs de protéines kinases (analyse)</term><term>Protéines de Saccharomyces cerevisiae (antagonistes et inhibiteurs)</term><term>Protéines à fluorescence verte (MeSH)</term><term>Saccharomyces cerevisiae (effets des médicaments et des substances chimiques)</term><term>Transduction du signal (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Protein Kinase Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Saccharomyces cerevisiae Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Inhibiteurs de protéines kinases</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Facteurs de transcription</term><term>Protéines de Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Saccharomyces cerevisiae</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Saccharomyces cerevisiae</term><term>Transduction du signal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Flow Cytometry</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cytométrie en flux</term><term>Humains</term><term>Protéines à fluorescence verte</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">TOR (target of rapamycin) is a serine/threonine kinase, evolutionarily conserved from yeast to human, which functions as a fundamental controller of cell growth. The moderate clinical benefit of rapamycin in mTOR-based therapy of many cancers favors the development of new TOR inhibitors. Here we report a high-throughput flow cytometry multiplexed screen using five GFP-tagged yeast clones that represent the readouts of four branches of the TORC1 signaling pathway in budding yeast. Each GFP-tagged clone was differentially color-coded, and the GFP signal of each clone was measured simultaneously by flow cytometry, which allows rapid prioritization of compounds that likely act through direct modulation of TORC1 or proximal signaling components. A total of 255 compounds were confirmed in dose-response analysis to alter GFP expression in one or more clones. To validate the concept of the high-throughput screen, we have characterized CID 3528206, a small molecule most likely to act on TORC1 as it alters GFP expression in all five GFP clones in a manner analogous to that of rapamycin. We have shown that CID 3528206 inhibited yeast cell growth and that CID 3528206 inhibited TORC1 activity both in vitro and in vivo with EC(50)'s of 150 nM and 3.9 μM, respectively. The results of microarray analysis and yeast GFP collection screen further support the notion that CID 3528206 and rapamycin modulate similar cellular pathways. Together, these results indicate that the HTS has identified a potentially useful small molecule for further development of TOR inhibitors.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22260433</PMID><DateCompleted><Year>2012</Year><Month>10</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1554-8937</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>4</Issue><PubDate><Year>2012</Year><Month>Apr</Month><Day>20</Day></PubDate></JournalIssue><Title>ACS chemical biology</Title><ISOAbbreviation>ACS Chem Biol</ISOAbbreviation></Journal><ArticleTitle>Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry.</ArticleTitle><Pagination><MedlinePgn>715-22</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/cb200452r</ELocationID><Abstract><AbstractText>TOR (target of rapamycin) is a serine/threonine kinase, evolutionarily conserved from yeast to human, which functions as a fundamental controller of cell growth. The moderate clinical benefit of rapamycin in mTOR-based therapy of many cancers favors the development of new TOR inhibitors. Here we report a high-throughput flow cytometry multiplexed screen using five GFP-tagged yeast clones that represent the readouts of four branches of the TORC1 signaling pathway in budding yeast. Each GFP-tagged clone was differentially color-coded, and the GFP signal of each clone was measured simultaneously by flow cytometry, which allows rapid prioritization of compounds that likely act through direct modulation of TORC1 or proximal signaling components. A total of 255 compounds were confirmed in dose-response analysis to alter GFP expression in one or more clones. To validate the concept of the high-throughput screen, we have characterized CID 3528206, a small molecule most likely to act on TORC1 as it alters GFP expression in all five GFP clones in a manner analogous to that of rapamycin. We have shown that CID 3528206 inhibited yeast cell growth and that CID 3528206 inhibited TORC1 activity both in vitro and in vivo with EC(50)'s of 150 nM and 3.9 μM, respectively. The results of microarray analysis and yeast GFP collection screen further support the notion that CID 3528206 and rapamycin modulate similar cellular pathways. Together, these results indicate that the HTS has identified a potentially useful small molecule for further development of TOR inhibitors.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Center for Molecular Discovery, University of New Mexico, Albuquerque, 87131, United States. juchen@salud.unm.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Young</LastName><ForeName>Susan M</ForeName><Initials>SM</Initials></Author><Author ValidYN="Y"><LastName>Allen</LastName><ForeName>Chris</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Seeber</LastName><ForeName>Andrew</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Péli-Gulli</LastName><ForeName>Marie-Pierre</ForeName><Initials>MP</Initials></Author><Author ValidYN="Y"><LastName>Panchaud</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Waller</LastName><ForeName>Anna</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Ursu</LastName><ForeName>Oleg</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>Tuanli</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Golden</LastName><ForeName>Jennifer E</ForeName><Initials>JE</Initials></Author><Author ValidYN="Y"><LastName>Strouse</LastName><ForeName>J Jacob</ForeName><Initials>JJ</Initials></Author><Author ValidYN="Y"><LastName>Carter</LastName><ForeName>Mark B</ForeName><Initials>MB</Initials></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Huining</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Bologa</LastName><ForeName>Cristian G</ForeName><Initials>CG</Initials></Author><Author ValidYN="Y"><LastName>Foutz</LastName><ForeName>Terry D</ForeName><Initials>TD</Initials></Author><Author ValidYN="Y"><LastName>Edwards</LastName><ForeName>Bruce S</ForeName><Initials>BS</Initials></Author><Author ValidYN="Y"><LastName>Peterson</LastName><ForeName>Blake R</ForeName><Initials>BR</Initials></Author><Author ValidYN="Y"><LastName>Aubé</LastName><ForeName>Jeffrey</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Werner-Washburne</LastName><ForeName>Margaret</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Loewith</LastName><ForeName>Robbie J</ForeName><Initials>RJ</Initials></Author><Author ValidYN="Y"><LastName>De Virgilio</LastName><ForeName>Claudio</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Sklar</LastName><ForeName>Larry A</ForeName><Initials>LA</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R03 MH086450</GrantID><Acronym>MH</Acronym><Agency>NIMH NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54HG005031</GrantID><Acronym>HG</Acronym><Agency>NHGRI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54 MH084690</GrantID><Acronym>MH</Acronym><Agency>NIMH NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R25 GM060201</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54 HG005031</GrantID><Acronym>HG</Acronym><Agency>NHGRI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54MH084690</GrantID><Acronym>MH</Acronym><Agency>NIMH NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R03 MH086450-01</GrantID><Acronym>MH</Acronym><Agency>NIMH NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54 HG005031-01</GrantID><Acronym>HG</Acronym><Agency>NHGRI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>U54 MH084690-01</GrantID><Acronym>MH</Acronym><Agency>NIMH NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>02</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ACS Chem Biol</MedlineTA><NlmUniqueID>101282906</NlmUniqueID><ISSNLinking>1554-8929</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D047428">Protein Kinase Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C561842">TORC1 protein complex, S cerevisiae</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005434" MajorTopicYN="N">Flow Cytometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D047428" MajorTopicYN="N">Protein Kinase Inhibitors</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>1</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>1</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22260433</ArticleId><ArticleId IdType="doi">10.1021/cb200452r</ArticleId><ArticleId IdType="pmc">PMC3331904</ArticleId><ArticleId IdType="mid">NIHMS354186</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Genes Dev. 2009 Aug 15;23(16):1929-43</Citation><ArticleIdList><ArticleId IdType="pubmed">19684113</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2008;3(5):e2223</Citation><ArticleIdList><ArticleId IdType="pubmed">18493323</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2008;3(2):e1663</Citation><ArticleIdList><ArticleId IdType="pubmed">18301741</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Pharmacol Sci. 2003 Sep;24(9):444-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12967766</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 2005 Aug;25(16):7239-48</Citation><ArticleIdList><ArticleId IdType="pubmed">16055732</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Biol. 2000 Dec 14-28;10(24):1574-81</Citation><ArticleIdList><ArticleId IdType="pubmed">11137008</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2011 Apr;22(7):988-98</Citation><ArticleIdList><ArticleId IdType="pubmed">21289090</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Soc Trans. 2009 Feb;37(Pt 1):242-7</Citation><ArticleIdList><ArticleId IdType="pubmed">19143640</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Oct 16;425(6959):686-91</Citation><ArticleIdList><ArticleId IdType="pubmed">14562095</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Pharmacol Sci. 2008 Oct;29(10):499-504</Citation><ArticleIdList><ArticleId IdType="pubmed">18755517</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biomol Screen. 1999;4(2):67-73</Citation><ArticleIdList><ArticleId IdType="pubmed">10838414</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Chem Biol. 2007 Jun;3(6):331-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17486044</ArticleId></ArticleIdList></Reference><Reference><Citation>Brief Funct Genomic Proteomic. 2007 Jun;6(2):81-90</Citation><ArticleIdList><ArticleId IdType="pubmed">17611236</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2001 Sep 14;276(37):34441-4</Citation><ArticleIdList><ArticleId IdType="pubmed">11457832</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biomol Screen. 2004 Mar;9(2):103-11</Citation><ArticleIdList><ArticleId IdType="pubmed">15006133</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2000 Nov 17;275(46):35727-33</Citation><ArticleIdList><ArticleId IdType="pubmed">10940301</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2001 Dec 17;20(24):7209-19</Citation><ArticleIdList><ArticleId IdType="pubmed">11742997</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1999 Dec 9;402(6762):689-92</Citation><ArticleIdList><ArticleId IdType="pubmed">10604478</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2000 Jun;11(6):2103-15</Citation><ArticleIdList><ArticleId IdType="pubmed">10848632</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Pharmacol. 2007 Oct;7(5):527-34</Citation><ArticleIdList><ArticleId IdType="pubmed">17652026</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2009;486:151-65</Citation><ArticleIdList><ArticleId IdType="pubmed">19347622</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Mol Cell Biol. 2011 Jan;12(1):21-35</Citation><ArticleIdList><ArticleId IdType="pubmed">21157483</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Signal. 2009;2(67):pe24</Citation><ArticleIdList><ArticleId IdType="pubmed">19383975</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2007 Jun 8;26(5):663-74</Citation><ArticleIdList><ArticleId IdType="pubmed">17560372</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2006 May;3(5):361-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16628206</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2002 Mar;13(3):795-804</Citation><ArticleIdList><ArticleId IdType="pubmed">11907262</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Biotechnol. 2010 Jul;28(7):738-42</Citation><ArticleIdList><ArticleId IdType="pubmed">20581845</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2003 Dec;12(6):1607-13</Citation><ArticleIdList><ArticleId IdType="pubmed">14690612</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 2010 Oct 1;21(19):3475-86</Citation><ArticleIdList><ArticleId IdType="pubmed">20702584</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Biochem Cell Biol. 2006;38(9):1476-81</Citation><ArticleIdList><ArticleId IdType="pubmed">16647875</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Chem Biol. 2004 Aug;8(4):392-8</Citation><ArticleIdList><ArticleId IdType="pubmed">15288249</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Med Chem. 2005;12(8):961-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15853708</ArticleId></ArticleIdList></Reference><Reference><Citation>Oncogene. 2006 Oct 16;25(48):6392-415</Citation><ArticleIdList><ArticleId IdType="pubmed">17041625</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2008 Jul;69(1):277-85</Citation><ArticleIdList><ArticleId IdType="pubmed">18513215</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 1999 Apr;10(4):987-1000</Citation><ArticleIdList><ArticleId IdType="pubmed">10198052</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Chem Biol. 2005 Feb;9(1):20-4</Citation><ArticleIdList><ArticleId IdType="pubmed">15701448</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 Dec 30;280(52):42528-35</Citation><ArticleIdList><ArticleId IdType="pubmed">16253991</ArticleId></ArticleIdList></Reference><Reference><Citation>Assay Drug Dev Technol. 2011 Feb;9(1):13-20</Citation><ArticleIdList><ArticleId IdType="pubmed">21050072</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5116-21</Citation><ArticleIdList><ArticleId IdType="pubmed">11309499</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Chem. 2011 Mar 10;54(5):1473-80</Citation><ArticleIdList><ArticleId IdType="pubmed">21322566</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Drug Discov. 2011 Nov;10(11):868-80</Citation><ArticleIdList><ArticleId IdType="pubmed">22037041</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Cell. 1996 Jan;7(1):25-42</Citation><ArticleIdList><ArticleId IdType="pubmed">8741837</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Biol. 2009 Feb 10;7(2):e38</Citation><ArticleIdList><ArticleId IdType="pubmed">19209957</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genomics. 2009 May;10(3):198-205</Citation><ArticleIdList><ArticleId IdType="pubmed">19881913</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Chem. 2010 Oct 14;53(19):7146-55</Citation><ArticleIdList><ArticleId IdType="pubmed">20860370</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Allen, Chris" sort="Allen, Chris" uniqKey="Allen C" first="Chris" last="Allen">Chris Allen</name><name sortKey="Aube, Jeffrey" sort="Aube, Jeffrey" uniqKey="Aube J" first="Jeffrey" last="Aubé">Jeffrey Aubé</name><name sortKey="Bologa, Cristian G" sort="Bologa, Cristian G" uniqKey="Bologa C" first="Cristian G" last="Bologa">Cristian G. Bologa</name><name sortKey="Carter, Mark B" sort="Carter, Mark B" uniqKey="Carter M" first="Mark B" last="Carter">Mark B. Carter</name><name sortKey="De Virgilio, Claudio" sort="De Virgilio, Claudio" uniqKey="De Virgilio C" first="Claudio" last="De Virgilio">Claudio De Virgilio</name><name sortKey="Edwards, Bruce S" sort="Edwards, Bruce S" uniqKey="Edwards B" first="Bruce S" last="Edwards">Bruce S. Edwards</name><name sortKey="Foutz, Terry D" sort="Foutz, Terry D" uniqKey="Foutz T" first="Terry D" last="Foutz">Terry D. Foutz</name><name sortKey="Golden, Jennifer E" sort="Golden, Jennifer E" uniqKey="Golden J" first="Jennifer E" last="Golden">Jennifer E. Golden</name><name sortKey="Kang, Huining" sort="Kang, Huining" uniqKey="Kang H" first="Huining" last="Kang">Huining Kang</name><name sortKey="Loewith, Robbie J" sort="Loewith, Robbie J" uniqKey="Loewith R" first="Robbie J" last="Loewith">Robbie J. Loewith</name><name sortKey="Panchaud, Nicolas" sort="Panchaud, Nicolas" uniqKey="Panchaud N" first="Nicolas" last="Panchaud">Nicolas Panchaud</name><name sortKey="Peli Gulli, Marie Pierre" sort="Peli Gulli, Marie Pierre" uniqKey="Peli Gulli M" first="Marie-Pierre" last="Péli-Gulli">Marie-Pierre Péli-Gulli</name><name sortKey="Peterson, Blake R" sort="Peterson, Blake R" uniqKey="Peterson B" first="Blake R" last="Peterson">Blake R. Peterson</name><name sortKey="Seeber, Andrew" sort="Seeber, Andrew" uniqKey="Seeber A" first="Andrew" last="Seeber">Andrew Seeber</name><name sortKey="Sklar, Larry A" sort="Sklar, Larry A" uniqKey="Sklar L" first="Larry A" last="Sklar">Larry A. Sklar</name><name sortKey="Strouse, J Jacob" sort="Strouse, J Jacob" uniqKey="Strouse J" first="J Jacob" last="Strouse">J Jacob Strouse</name><name sortKey="Ursu, Oleg" sort="Ursu, Oleg" uniqKey="Ursu O" first="Oleg" last="Ursu">Oleg Ursu</name><name sortKey="Waller, Anna" sort="Waller, Anna" uniqKey="Waller A" first="Anna" last="Waller">Anna Waller</name><name sortKey="Werner Washburne, Margaret" sort="Werner Washburne, Margaret" uniqKey="Werner Washburne M" first="Margaret" last="Werner-Washburne">Margaret Werner-Washburne</name><name sortKey="Yao, Tuanli" sort="Yao, Tuanli" uniqKey="Yao T" first="Tuanli" last="Yao">Tuanli Yao</name><name sortKey="Young, Susan M" sort="Young, Susan M" uniqKey="Young S" first="Susan M" last="Young">Susan M. Young</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Chen, Jun" sort="Chen, Jun" uniqKey="Chen J" first="Jun" last="Chen">Jun Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001181 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001181 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22260433
|texte= Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22260433" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |